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. 2018 Nov 18;11(11):1581–1586. doi: 10.14202/vetworld.2018.1581-1586

Table-1.

E. coli-resistant genes and primer sequences used for single PCR identification.

Antibiotics Resistance gene Primer sequence Size (bp) Annealing temperature Reference
Tetracycline tet (A) (F) 5′-GGTTCACTCGAACGACGTCA-3′ 577 57°C [14]
(R) 5′-CTGTCCGACAAGTTGCATGA-3′
tet (B) (F) 5′-CCTCAGCTTCTCAACGCGTG-3′ 634 56°C [14]
(R) 5′-GCACCTTGCTCATGACTCTT-3′

PCR=Polymerase chain reaction, E. coli=Escherichia coli