Figure 4.

Fecundity of females with gene knockdown. (a) Number of eggs produced by females treated with dsGfp (n = 19), dsNlHdac1 (n = 19), dsNlHdac4 (n = 19), dsNlHdac6 (n = 16), dsNlHdac11 (n = 18) or dsNlHdac11_like (dsNlHdac11_l) (n = 19). (b) Number of eggs produced by females treated with either dsGfp (n = 17), or dsNlHdac3 (n = 19). (c) Number of eggs produced by females treated with either dsGfp (n = 20), dsNlSirt1 (n = 20), dsNlSirt2 (n = 20), dsNlSirt3 (n = 20), dsNlSirt4 (n = 20), dsNlSirt6 (n = 20) or dsNlSirt7 (n = 20). Females were allowed to deposit eggs for 5 days in (b), and for 10 days in (a) and (c). Each circle represents eggs produced by an individual female. Bar represents mean ± s.e.m. Statistical comparisons between two groups were performed using two-tailed Student's t-test (*p < 0.05; **p < 0.01).