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. 2018 Dec 22;18:1283. doi: 10.1186/s12885-018-5204-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Overexpression of shRNA-resistant Sema5A rescues epithelial phenotype. a-b Western blot analysis is showing an increase in SEMA5A and decrease in β-catenin expression in T3M-4-shSEMA5A (a) and CD18/HPAF-shSEMA5A cells (b) transfected with control pBK-CMV vector and full-length mouse Sema5A. GAPDH was used as loading control. c Increase in E-Cad and decrease in N-cad protein expression with respect to β-actin as a loading control in T3M-4-shSEMA5A cells transfected control pBK-CMV vector and full-length mouse Sema5A. d Increase in E-Cad and decrease in Snail protein expression with respect to GAPDH as a loading control in CD18/HPAF-shSEMA5A cells transfected control pBK-CMV vector and full-length mouse Sema5A. The intensity of the bands in Western blot analysis was quantified using Image J software and was normalized with respect to the T3M-4-shSEMA5A or CD18/HPAF-shSEMA5A cells transfected with control pBK-CMV vector