Fig. 5.

Heterologous expression of the ThPI paralogs in A. thaliana. a a wildtype (WT) A. thaliana flower. b a 35S::AtPI flower, which shows the phenotype obtained by constitutive expression of the native PI. Note the change in orientation of the first whorl organs. c a 35S::ThPI-1 flower, showing homeotic conversion of sepals to petals. d a 35S::ThPI-2 flower, showing no aberrant phenotype. Top row shows whole flower, bottom row shows dissected sepals (top) and petals (bottom). e AtPI expression levels in 35S::AtPI lines. f ThPI-1 expression levels in 35S::ThPI-1 lines. g ThPI-2 expression levels in 35S::ThPI-2 lines. h ThPI-2 expression levels in additional 35S::ThPI-2 lines. Lines that showed an overexpression phenotype are indicated with an asterisk. Expression measured in leaves, and calculated relative to a reference gene (TIP41). For the expression in e, f and g, Rosette leaves of 6–8 week plants were used. In h, the first developing rosette leaves were used