Table 1.

Primers used for the 3’RACE and RNA in situ hybridisation. Primers to obtain the 3’UTR sequence as well as to generate the in situ RNA probes are shown

In situ probes	Fw	R
ThAP3	CTCTCCATTCTCTGCGACGCTAG	CATCAAGCTAGGTTTTTCAACTCC
ThPI-1	GCTCTCCTTCAATGGATCTTGGTG	CACTTATGTCCAAGTCCTTGCAGAG
ThPI-2	GATCACTGTTCTATGCGACGCC	GAAACACGCAACGAACCTTGTC
3’ RACE	First PCR	Nested PCR
ThAP3–1	CTCACTACGAAAGGATGCAAGAGAC	GAAGTTTAAATCGATTGGCAGCC
ThAP3–2	CCTCTCACTACGAAAGGATGCAG	CGATTGGCAATAAAATTGAAACC
ThPI-1	GAGCAGTATCAAAGGATCGCC	GGCCATAGAGCACGCAGTCC
ThPI-2	GAGATGTTGGGCACTTATCAGC	CAAAAGCCTAATCGCCATAGAGAG