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. 2018 Dec 22;18:368. doi: 10.1186/s12870-018-1574-0

Table 2.

Primers used to generate pSPUTK constructs used for in vitro protein production

pSPUTK cloning
Gene Fw R
AP3–1/2 GATAGATCTATGACGAGGGGAAAGATTCAG GATAGATCTTCATTCGAGCAAGTGGAAGG
ThPI-1 TTACCATGGGGAGAGGAAAGATAGAG ATTATCGATCAGTCGATGACCAAAGACATGATC
ThPI-2 AATCCATGGGAAGAGGGAAGATAGAGATCAAAAG TTTATCGATCAGACGATGTGTTGTAAATTGGGC
Th2954 (AG) ACGGCGTACCAAACGGAGTTG TTACACTAACTGAAGTGGAGTGTG
Th1528 (SEP3) CATGCCATGGGAAGAGGTCGTGTTGAG AAGATCGATCAATTGTTGTCATAAGGTAACCAAC
Th18678 (SEP3) CATGCCATGGGGAGAGGTCGAGTTG AAGATCGATCAATTGTTGTCGTAAGGTAACCAAC
Th21984 (SEP4) ATGGGAAGAGGGAAAGTGGAGC TCAGATCATCCAGCCGTGGAA
Th2854 (SEP1/2) ATGGGGAGGGGTAGGGTTG TCAGAGCATCCAACCAGGG
Th13754 (AP1) ATGGGAAGGGGAAGGGTTCAG TTATGTGAAGCAGCCAAGGTTGCAATC