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. 2018 Dec 22;12:35. doi: 10.1186/s13036-018-0129-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — CD24⁺ NP cells express a higher level of notochordal/immature NP cell marker. a-c Immunofluorescence staining analysis of brachyury, SHH and GLUT-1 in CD24⁺, CD24⁻ and unsorted NP cells. d-e Western blot analysis of KRT8, brachyury, SHH and GLUT-1 expression in CD24⁺, CD24⁻ and unsorted NP cells. *P < 0.05, **P < 0.01, ***P < 0.001. P-values were analyzed by t one-way ANOVA. Scale bars represent 50 μm