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. 2018 Dec 22;12:35. doi: 10.1186/s13036-018-0129-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — CD24⁺ NP cells exhibit a higher proliferation rate and higher osteogenic/adipogenic/chondrogenic differentiation ability than CD24⁻ and unsorted NP cells in vitro. a The proliferative activity of CD24⁺, CD24⁻ and unsorted NP cells detected by CCK8. b-c Osteogenic differentiation of CD24⁺, CD24⁻ and unsorted NP cells analysed by alkaline phosphatase (b) and alizarin red staining (c). d Adipogenic differentiation of CD24⁺, CD24⁻ and unsorted NP cells analysed by oil red O staining. e Chondrogenic differentiation of CD24⁺, CD24⁻ and unsorted NP cells analysed by alcian blue staining. **P < 0.01, ***P < 0.001. P-values were analyzed by one-way ANOVA