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Western blot analysis of the periplasm (PP) and spheroplast (SP) samples from shaker flask induction. a–d Precursor protein processing and spheroplast retention of scFvβ and scFvT were assessed with either DsbA E1 or Piii E5 signal peptide. e–h The same periplasm fractions were also assessed for the disulfide bond formation under reducing and non-reducing conditions, with (+) or without (−) DTT
