Table 1.
Expression performance of the BL21(LV2)-pENTRY strains containing the codon optimised signal peptide sequences
| Signal peptide | Max (IP) | IPTG (I) | Uninduced (UI) | IP/I | IP/UI | PPDA concentration (μM) at max (IP) | OD600 at max (IP) | |
|---|---|---|---|---|---|---|---|---|
| SRP-dependent | DsbA WT | 1629 | 754 | 64 | 2 | 26 | 8 | 1 ± 0 |
| DsbA E1 | 2152 | 202 | 65 | 11 | 33 | 400 | 18 ± 5 | |
| yBG L2 WT | 4017 | 1283 | 75 | 3 | 54 | 8 | 11 ± 10 | |
| yBGL2 H1 | 4493 | 336 | 75 | 13 | 60 | 200 | 20 ± 4 | |
| SecB-dependent | Piii WT | 17 | 21 | 34 | 1 | 0 | 400 | 14 ± 6 |
| Piii E5 | 9818 | 607 | 77 | 16 | 127 | 400 | 24 ± 3 | |
| PelB WT | 1297 | 179 | 74 | 7 | 18 | 400 | 21 ± 2 | |
| PelB A5 | 2913 | 355 | 76 | 8 | 38 | 100 | 16 ± 6 |
Gene expression monitored as relative fluorescence units (RFU) over optical cell density (OD) in the absence of inducer (UI), with IPTG induction only (I) and with both IPTG and PPDA induction (IP)