Table 2.
scFvβ expression and periplasmic secretion titres with DsbA E1 and Piii E5 signal peptides in the BL21(LV2)-pDEST and BL21(DE3)-pET28 strains
| scFvβ | ||||
|---|---|---|---|---|
| Signal peptide | DsbA-E1 | Piii-E5 | ||
| BL21 strain | (LV2) | (DE3) | (LV2) | DE3 |
| Expression titre mg/L |
36.7 ± 10.4 | 25.3 ± 3.6 | 101.7 ± 31.1 | 6.2 ± 0.5 |
| Induction control (max/basal) |
≥ 3666 | 17 | ≥ 10,165 | 3 |
| Secretion titre mg/L |
26.2 ± 7.3 | 4.5 ± 0.7 | 17.0 ± 0.2 | 1.9 ± 0.3 |
| OD600 at max | 11.0 ± 0.3 | 3.7 ± 0.3 | 10.0 ± 0.5 | 1.5 ± 0.0 |
Performed in shaker flask induced for 14 h at 30 °C. No basal expression was observed in the BL21(LV2) strains, within the western blot detection limit. Induction control (max/basal) was calculated relative to the lower detection limit