Figure 3.

Larval dsx and smo expression levels in control-injected individuals and following Foxo^RNAi, InR1^RNAi, InR2^RNAi and InR1 + 2^RNAi. (a) qRT-PCR using whole-body larvae shows that dsx expression levels are unaffected by Foxo^RNAi or InR1^RNAi, but significantly reduced following InR2^RNAi and marginally significantly reduced following InR1 + 2^RNAi. This suggests that InR2, but not InR1, promotes dsx expression in wild-type individuals. (b) smo expression levels are significantly reduced following Foxo^RNAi, but unaffected by InR1^RNAi, InR2^RNAi and InR1 + 2^RNAi, suggesting that Foxo promotes smo expression in wild-type individuals. Box plots show 25% and 75% quartiles (boxes), medians (lines dividing the boxes), outermost values (whiskers), outliers (dots) and data points (dots overlapping with boxes and whiskers). *p < 0.05, ^•p < 0.1.