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. 2018 Dec 15;10(12):476–486. doi: 10.4251/wjgo.v10.i12.476

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©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Tumor tissue sections at 1 d and 7 d after the irreversible electroporation treatment were stained for Ki67 and cleaved caspase-3. A: A representative immunohistochemistry image in an untreated pancreatic parenchyma; B: In tumor tissues, extensive caspase-3 activation was observed on the first postoperative day. Irreversible electroporation significantly increased cell proliferation (Ki67 staining) at 1 d post-treatment, but cell proliferation was decreased at 7 d post-treatment (arrows); C: Limited caspase-3 staining at 7 d post-irreversible electroporation treatment was found in treated tumors, while most of the viable tumor tissues showed no caspase-3 activation. The slides were imaged at 400 × by light microscopy.