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. 2018 Dec 17;9:1473. doi: 10.3389/fphar.2018.01473

FIGURE 6.

FIGURE 6

In vitro Experimental validation. (A) Cell viability of RAW264.7 and H1975 cells. The determination of cell viability of RAW264.7 and H1975 cells was carried out by CCK-8 assay after treated with control or baicalein for 48 h. (B) RAW264.7 cells were pretreated with (5, 15, or 25 μM), or the combinations for 2 h, vehicle as the control. The exposure to LPS (1 μg/ml) for 20 h, then iNOS, NF-κB and COX-2 accumulation of cytoplasm were measured by western blot. GAPDH was used as loading control. (C) H1975 cells were treated with baicalein (5, 10, or 15 μM) for 2 h, next, cell lysates were used to assess the phosphorylation of ERK, p38, and AKT. The total ERK, p38, and AKT are shown as a loading control. (D) Western blot analysis of CDK2, Bax, and Bcl-2 expression in H1975 cells. Cells were treatment with or without baicalein (5, 10, 15, or 20 μM) for 24 h. All results are repeated at least three independent experiments with the same tendency.