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. 2018 Dec 17;9:1473. doi: 10.3389/fphar.2018.01473

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Copyright © 2018 Liu, Jiang, Li, Zhang, Li, Hao, Su, Zhu, Zheng, Xiao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo Experimental validation, tumor samples were isolated from LLC-bearing mice at the end of the study and processed for flow cytometry analysis. (A) Percentage of CD8+ Granzyme B+ and CD8+ IFNγ+ T cells of CD45+ cells were measured by Flow cytometry, and protein expression shown as mean fluorescence intensity (MFI). (B) Flow cytometry analysis of tumor samples shows the effect of control and baicalein treatment on Foxp3 levels. (C) FACS analysis of tumor-cell suspensions from LLC-bear mice after control or baicalein treatment. Quantification of NK cell present in the tumor samples. Results are shown as mean ± SEM (^∗∗p < 0.01; ^∗∗∗p < 0.001).