FIGURE 5.
Validation of expression profile of selected miRs in liver MNCs. We selected two miRs (miR-130a and miR-185) based on their ability to target CSF1 as suggested in figure for further validation. (A) Seed sequence and alignment of the miR-130a and miR-185 with 3′ UTR of CSF1 gene. (B) Using mouse miR-specific primers for mature miR-130a and miR-185, Real-Time PCR was performed to determine their expression in liver MNCs. SNORD96a was used as an internal control. Data presented for both miR-130a and miR-185 represent the mean ± SEM of three independent experiments (B). Asterisk (∗) indicates statistically significant (p < 0.05) difference between the groups compared.