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. 2018 Dec 17;9:2922. doi: 10.3389/fimmu.2018.02922

Figure 5.

Figure 5

Blockade of LAG-3 following UV-B induced HSV-1 reactivation reduces recurrent ocular herpes. (A) Schematic representation of UV-B induced reactivation, blockade by mAb treatment, virological and immunological analyses, and recurrent eye disease scoring in 40 B6(Cg)-Tyrc−2J/J mice (6–8 weeks old) categorized into α-LAG-3 + gB498−505, α-LAG-3 alone, gB498−505 alone, and mock groups on the basis of different immunization strategies following ocular infection on day 0 with 1 × 106 pfu of HSV-1 (strain McKrae). (B) Cumulative reactivation score till 30-day post UV-B. (C) The cumulative number of eyes showing recurrent disease till 30-day post UV-B. (D) Average of total eye disease score of each day till 30-day post UV-B. (E) Average viral shedding evaluation by plaque assay after the last day of LAG-3mAb treatment (day 8 post UV-B). (F) Representative eye pictures of recurrent disease. Results are representative of two independent experiments. The indicated P-values, calculated using the unpaired t-test, show statistical significance between α-LAG-3 + gB498−505 and other groups.