Figure 5.

TLR7 Activation by PLA in Muscle Tissue

Representative images of TLR7-IRAK4 PLA (white) 6 hr following i.m. injection into the anterior tibialis of 10 μg luciferase IVT mRNA (red) unformulated or formulated with CholK. Extended focus images are shown. Scale bars, 20 μm (full images) and 5 μm (crops). White boxes show cropped selection. Quantification of TLR7-IRAK4 PLA is shown. Statistics were performed with a two-way ANOVA with a Tukey’s multiple comparisons test, where n = 30, except for sham 1.5 hr where n = 12, and *p < 0.04 and ***p < 0.0004. 95% confidence intervals are shown in black.