Figure 8.

PRR Activation by PLA in Lymph Node Tissue

Lymph nodes were excised and fixed 16 hr following i.m. injection into the anterior tibialis of 10 μg luciferase IVT mRNA (red) unformulated or formulated with CholK. (A) Representative images of TLR7-IRAK4 PLA (white) shown. Extended focus images are shown with 10-μm scale bars. Quantification of TLR7-IRAK4 PLA is shown. Statistics were performed with a one-way ANOVA with a Dunn’s multiple comparisons test, where n = 30 and *p < 0.02 and ***p < 0.0002. 95% confidence intervals are shown in red. (B) Representative images of RIG-I-MAVS PLA (white) shown. Extended focus images are shown with 10 μm scale bars. Quantification of RIG-I-MAVS PLA is shown. Statistics were performed with a one-way ANOVA with a Dunn’s multiple comparisons test, where n = 30. 95% confidence intervals are shown in red. (C) Representative images of MDA5-MAVS PLA (white) shown. Extended focus images are shown with 10 μm scale bars. Quantification of MDA5-MAVS PLA is shown. Statistics were performed with a one-way ANOVA with a Dunn’s multiple comparisons test, where n = 30, except for the mRNA + CholK condition where n = 18, and ****p < 0.0001. 95% confidence intervals are shown in red.