Figure 4.

Improved survival and histopathological measures in GBM CNS-1 model rats treated by SIXAC. (A) Kaplan-Meier curves comparing saline treated rats (circles) to groups of high (triangles) and moderate (squares) dose of SIXAC treated rats. There was a significant trend of prolonged survival in comparing the three groups (log-rank test for trend; p = 0.030). The effect of the moderate dose treatment on survival was more pronounced (log-rank test; p = 0.01) than the effect of the low dose (p < 0.05) in comparison to the saline treated group. PM brain tumor volumes in correlation to survival in days. Linear regression lines and Pearson R² values are shown for each group. (B) Control group, (C) Moderate dose treated group, (D) High dose treated group. Number of animals: control = 15, SIXAC moderate dose = 16, SIXAC high dose = 14. (E) Representative H&E brain stainings of control, SIXAC, and “long survivors” groups (magnification 10X). White arrows indicate mitosis (magnification 400X). (F) Representative Ki67 immunostaining of control and SIXAC group. (G) Number of mitosis/field as was calculated from H&E staining. (H) Ki67 index as was calculated using the ImmunoRatio application. Results are presented as mean ± SEM. *p < 0.05, ***p < 0.001.