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. 2018 Dec 17;9:647. doi: 10.3389/fgene.2018.00647

FIGURE 1.

FIGURE 1

Characterization of the hsa_circ_0043813 circRNA deriving from the STAT3 gene. (A) Schematic representation of the STAT3 genomic region spanning from exon 12 to 14. Exons are depicted as boxes (in scale), and introns as lines. The position of the SNP rs2293152 is shown by an arrow. (B) Schematic representation of the formation of the STAT3 circRNA hsa_circ_0043813 through a back-splicing event between exons 14 and 12. Exons are approximately drawn to scale; the curved arrow joins the 5′ splice site of exon 14 to 3′ splice site of exon 12. On the right, a schematic representation of the circRNA is depicted; arrows below exon 12 and 14 indicate the divergent primer couple used to detect the circRNA. Below the scheme, direct-sequencing electropherogram shows the head-to-tail splice junction, indicated by a black arrow, located between exons 14 and 12. (C) Boxplots showing expression levels of the hsa_circ_0043813 circRNA measured by semi-quantitative real-time RT-PCR in PBMCs of 35 healthy controls. Boxes define the interquartile range; the thick line refers to the median. Results were normalized to expression levels of the HMBS housekeeping gene, and for each sample three technical replicates were performed. The number of subjects belonging to each group is also indicated (n). The significance level of t-test analysis is shown. ∗p < 0.05; ns, not significant.