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. 2018 Dec 17;9:2953. doi: 10.3389/fimmu.2018.02953

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Copyright © 2018 Bak, Tischer, Dragon, Ravens, Pape, Koenecke, Oelke, Blasczyk, Maecker-Kolhoff and Eiz-Vesper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sirolimus has no effect on the dynamics of TCR repertoires. Isolated CD8⁺ T cells were expanded for 7 days on aAPCs and treated with or without sirolimus in the presence of IL-2. On day 7, CMV-specific CD8⁺ cells were sorted based on their multimer-specificity (A02pp65_p) by high speed flow cytometry sorters and subjected to high throughput TCR repertoire analysis. (A) The highest expanded CDR3 clones in two donors are shown as percentages (%) of productive reads. (B) Numbers of the CDR3 clones were normalized to 5,000 reads. (C) The Shannon index indicates the diversity of the repertoires, shown as normalized values. Values are displayed as mean (±) SD. Statistical analysis: two-way analysis of variance [non-significant (ns)].