Figure 6.

Incomplete mTORC1 inhibition has differential effects on the phosphorylation of signaling molecules. Total CD8⁺ T cells were re-stimulated with 10 μg/ml A02pp65_p for 1 h following 7 days of aAPC stimulation with or without sirolimus in the presence of IL-2, and the percentages (%) of phosphorylated (A) S6, (B) ERK1/2, (C) Akt^Thr308, (D) Akt^Ser473, and (E) STAT-5 were determined by phospho-flow cytometry. Values are displayed as mean (±) SD. The two-paired Student's t-test was used to test for statistically significant differences [***p < 0.001, non-significant (ns)].