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GtACR1 activation can inhibit ventricular myocyte action potentials in isolated zebrafish hearts. In a subset of 3-months post fertilization (mpf) isolated zebrafish hearts (n = 3/7), a 0.16 mm2 spot of 531/22 nm light applied to some eGFP expressing regions locally inhibited AP by depolarization of membrane potential, while there was no effect in regions with no eGFP expression (confirmed by live confocal microscopy).
