Table 2.

Composition of solutions used for cell isolation.

	Normal Tyrode solution	Ca2+- free cardioplegic solution	Enzyme solution	Blocking solution
NaCl [mM]	137	137	137	137
KCl (VWR International bvba, Leuven, Belgium) [mM]	4	14	14	14
HEPES [mM]	10	10	10	10
Creatine [mM]	10	10	10	10
Taurine [mM]	20	20	20	20
Glucose [mM]	10	10	10	10
MgCl2 [mM]	1	1	1	1
Adenosine [mM]	5	5	5	5
L-carnitine [mM]	2	2	2	2
CaCl2 (Honeywell FlukaTM, Seelze, Germany) [mM]	1	-	0.1	0.1
Heparin-sodium [units/L]	5,000	-
EGTA (Carl Roth GmbH, Karlsruhe, Germany) [mM]		0.096	0.096	0.096
Collagenase type 2, 315 U/mg (Worthington, Lakewood, NJ, USA) [g/L]			0.6
Protease XIV [g/L]			0.03
Bovine serum albumin [%]				0.5

All solutions were adjusted to pH 7.4 at 37°C.