Table 3.
Composition of bath solutions used in whole-cell patch-clamp experiments.
| Components of external bath solution [mM] | Standard solution | Alkaline solution | Strongly reduced Na+ concentration | Moderately reduced Na+ concentration | Strongly reduced Cl− concentration | Moderately reduced Cl− concentration | Moderately increased K+ concentration | Strongly increased K+ concentration |
|---|---|---|---|---|---|---|---|---|
| NaCl | 140 | 140 | 1 | 12 | - | 60 | 140 | 140 |
| Na-aspartate | - | - | - | - | 140 | 80 | - | - |
| Tetraethyl-ammoniumchloride | - | - | 139 | 127 | - | - | - | - |
| K-aspartate | - | - | - | - | - | - | 6.6 | 21.6 |
| KCl | 5.4 | 5.4 | 5.4 | 5.4 | 5.4 | 5.4 | 5.4 | 5.4 |
| CaCl2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| MgCl2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| Glucose | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| HEPES/TRIS | 10/- | -/10 | 10/- | 10/- | 10/- | 10/- | 10/- | 10/- |
| pH | 7.4 | 9 | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 | 7.4 |