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Composition of pipette solutions used in whole-cell patch-clamp experiments.
| Components of internal (pipette) solutions [mM] | Standard solution | Strongly reduced Cl− concentration | Moderately reduced Cl− concentration |
|---|---|---|---|
| KCl | 50 | - | 11 |
| K-aspartate | 80 | 130 | 119 |
| MgCl2 | 2 | 2 | 2 |
| Mg-ATP | 3 | 3 | 3 |
| EGTA | 10 | 10 | 10 |
| HEPES | 10 | 10 | 10 |
All solutions were adjusted to pH 7.2.
