Effects of Astilbe chinensis Franch. et Savat. (AC) extract on epididymal fat size in HFD induced obese mice. (a) The H & E stained epididymal fat tissue from HFD induced obese mice treated with RD or AC (100 and 200 mg/kg), and (b) quantification of epididymal fat tissues size were calculated. The (c) mRNA levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1, as determined by reverse transcription PCR (upper panels) and real-time PCR (lower panels), and (d) the protein levels of PPAR-γ, C/EBPα, SREBP1, FAS, and SCD-1 were examined by western blot analyses, respectively. ###p< 0.001 vs RD mice; ∗p< 0.05, ∗∗p< 0.01, and ∗∗∗p< 0.001 compared with HFD mice.