FIG 1.

Transposon mutagenesis screen identifies biofilm mutants with altered colony and pellicle biofilm formation. (A) Colony biofilms of parental and lon::Tn strains on BHI Congo red plates. (B) Biofilm pellicles formed in BHI medium in a 12-well untreated polystyrene dish and imaged at 48, 72, and 96 h. Panels A and B show representative images, at 96 h, of experiments performed in at least triplicate. (C) Absorbance at 570 nm for crystal violet staining, at 96 h, of polystyrene-adhered cells from pellicle biofilms formed in BHI medium. The data are representative data for experiments performed in triplicate. Error bars represent standard deviations. An unpaired two-tailed t test was used for statistical analysis, relative to the parental strain. *, P < 0.05.