FIG 2.

Lon protease negatively regulates biofilm formation in A. baumannii. (A) Colony biofilms of WT, lon::FRT, and lon::FRTc strains on BHI Congo red plates. (B) Pellicle biofilms of the same strains as in panel A in BHI medium. Panels A and B show representative images, at 96 h, of experiments performed in at least triplicate. (C) Absorbance at 570 nm for crystal violet staining, at 96 h, of polystyrene-adhered cells from pellicle biofilms formed in BHI medium. The data are representative data for experiments performed in triplicate. Error bars represent standard deviations. An unpaired two-tailed t test was used for statistical analysis, relative to the WT strain. *, P < 0.05; NS, not significant.