FIG 2.

SigB regulates biofilm growth. (A) Rate of biofilm (floating pellicle) formation by wild-type (wt) JH642 and isogenic ΔsigB cells grown in static LBY broth. Inset photographs are representative of typical biofilms developed for 50 h and stained with crystal violet. (B) β-Galactosidase activity of the biofilm-reporter fusion epsG-lacZ::amyE in wild-type and isogenic ΔsigB cells grown in static LBY broth. A typical output of three independent experiments performed in parallel is shown. (C) β-Galactosidase activity of the biofilm-reporter fusion bslA-lacZ::amyE in wild-type and isogenic ΔsigB cells grown in static LBY broth. Each data point is the mean ± SEM. A typical output of three independent experiments performed in parallel is shown.