Fig. 1.

PluTo transgene design and male phenotype. a The PluTo transgene is composed of 2688 bp of the 5' regulatory region immediately upstream of the start codon of AGAP009368 followed by Plugin cDNA fused to a 6-serine linker followed by tdTomato. The transgene also carries a 3xP3-EYFP selectable marker cassette for identification of transgenic larvae. Each coding sequence has an SV40 terminator to prevent transcriptional read-through. Following insertion, the flanking recombination sites attL and attR are generated in the genome. b Adult PluTo transgenic males display strong tdTomato fluorescence in their MAGs (lower panels). The fluorescence is clearly and easily identifiable under a fluorescent dissecting microscope through the adult male cuticle (upper panels). Scale-bar: 100 μm