Figure 3.

Systemic depletion of kallistatin exacerbates aortic oxidative stress and inflammation. (a) Representative images of superoxide formation in aortas from WT, KS^−/− mice with or without STZ treatment, as indicated by red fluorescence dye DHE. Collagen fibers displayed green autofluorescence in the aorta. (b) NADPH oxidase in mouse aorta. (c) Representative western blot of SIRT1 and eNOS expression in mouse aorta. (d&e) Inflammatory gene expression of TNF-α and ICAM-1 in mouse aorta. Values are expressed as mean ± SEM. n = 3. ^∗P < 0.05 vs. WT group, ^#P < 0.05 vs. WT + STZ group.