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. 2018 Dec 3;2018:7283703. doi: 10.1155/2018/7283703

Figure 5.

Figure 5

MSCs attenuate neutrophil recruitment via hindering CXCL2/CXCR2 signaling. (a, e) Surface expression of CXCR2 on neutrophils was detected by flow cytometry analysis. (b) The mean fluorescence intensity of CXCR2 on the surface of neutrophils was recorded. (c) The levels of p38 MAPK phosphorylation in neutrophils were detected by flow cytometry analysis, and (d) the consequences were verified by Western blot analysis. (f) The expression levels of mRNA of various chemokines involved in hepatic IRI were analyzed by qRT-PCR. Fold change represents the expression of each chemokine in I/R liver lobes 12 h postreperfusion compared with normal liver. (g) Macrophages were cultured in the presence or absence of MSCs for 12 h. The concentration of CXCL2 in the supernatant was measured by ELISA. (h) The levels of NF-κB p65 phosphorylation in macrophages were detected by flow cytometry analysis. Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. p-P38: phospho-p38 MAPK; p-P65: phospho-NF-κB p65.