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. 2018 Dec 3;2018:8092713. doi: 10.1155/2018/8092713

Figure 3.

Figure 3

Treatment α-tocopherol and γ-tocopherol inhibits the nitrite production and expression of iNOS and COX-2 against LPS-activated microglia cells. BV2 microglial cells were activated with LPS (100 ng/mL) 30 min before sample treatment, and samples of different concentrations were added under the same condition. Incubation of LPS and sample was performed for 6 h for iNOS and COX-2 expression and 24 h for nitrite production assay. (a, b) Nitrite production and cell viability of LPS-activated BV2 microglial cells after treatment of α-tocopherol and γ-tocopherol (c–e) iNOS and COX-2 expression and their respective band intensity. Tubulin was used as loading control. All data are presented as the mean ± standard error of the mean of three independent experiments. ∗∗P < 0.01 and ∗∗∗P < 0.001 indicate significant differences compared with LPS treatment alone. ###P < 0.001 indicates significant differences compared with untreated control group. Ctl, untreated control and LPS, lipopolysaccharide.