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. 2018 Dec 3;2018:8092713. doi: 10.1155/2018/8092713

Figure 6.

Figure 6

Treatment with resveratrol-enriched rice inhibits NF-κB translocation and I-kB phosphorylation in lipopolysaccharide-activated BV2 cells. BV2 microglial cells were pretreated with normal rice (NR), resveratrol-enriched rice (RR), and resveratrol after 30 min of LPS (100 ng/mL) stimulation. NF-κB and I-kB/pI-kB expression was determined after 1 h of LPS activation. (a, b) Cytosolic NF-κB expression and band intensity. β-Actin was used as loading control. (c, d) Nucleolar NF-κB expression and band intensity. Histone-3 was used as loading control. (e, f) Cytosolic I-kB and pI-kB expression and band intensity. β-Actin was used as loading control. All data are presented as the mean ± standard error of the mean of three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 indicate significant differences compared with LPS treatment alone. #P < 0.05 and ###P < 0.001 indicate significant differences compared with untreated control group. $P < 0.05 and $$$P < 0.001 indicate significant differences to RR compared to NR. Ctl, untreated control and LPS, lipopolysaccharide.