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. 2018 Dec 3;115(51):E12015–E12023. doi: 10.1073/pnas.1717944115

Fig. 6.

Fig. 6.

Crystal structure of NSP2 S313D shows strong interoctameric hydrogen bond interactions. (A) Top and side views of the crystal structure of the NSP2 S313D phosphomimetic mutant (PDB ID code 6AUK) showing the interaction between two NSP2 octamers. Each NSP2 monomer is shown in a different color. The black box outlines the magnified view of the four interoctamer interactions formed between R287 and D313. (B) Serine phosphomimetic mutant S313D hydrogen bond interaction between D313 and R287 (2.7 Å) (Upper) compared with modeled phosphorylated S313 that can engage in two hydrogen bond interactions with R287 (3.0 Å and 2.8 Å) (Lower) demonstrating phosphorylated S313 can facilitate interoctamer interaction as observed in the crystal structure of NSP2 S313D. (C) Magnified view of NSP2 S313A crystal structure (PDB ID code 6CYA) showing A313 is positioned too distantly from R287 (4.0 Å) to form interoctamer interactions.