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Simulations demonstrate the extendibility of multicolor fm-DNA-PAINT to five-color channels. Demodulated frames for each channel from a five-color synthetic image generated with camera frame rate F = 100 Hz and fi of 50, 40, 30, 20, and 10 Hz, assuming that the fluorophores have minimal spectral overlap (similar to the two-color experimental data with Cy5 and Cy3). The overlapped (combined) image is shown in the lower right.
