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. 2018 Dec 3;115(51):12991–12996. doi: 10.1073/pnas.1804725115

Fig. 3.

Fig. 3.

Simulations demonstrate the extendibility of multicolor fm-DNA-PAINT to five-color channels. Demodulated frames for each channel from a five-color synthetic image generated with camera frame rate F = 100 Hz and fi of 50, 40, 30, 20, and 10 Hz, assuming that the fluorophores have minimal spectral overlap (similar to the two-color experimental data with Cy5 and Cy3). The overlapped (combined) image is shown in the lower right.