Fig. 2.

In vitro culture of adrenocortical progenitors. (A) Adrenocortical cells of Nestin-GFP mice were isolated and cultured under low-attachment conditions. On day 6, the culture conditions were changed and differentiation was induced. The expression of GFP was tracked throughout the experiment, and representative images are shown. (B) qRT-PCR showing the relative expression of various stem cell markers and (C) steroidogenic markers plus the ACTH receptor at 3-d intervals following isolation and culture. Data in B and C are presented as mean ± SEM (n ≥ 3). *P < 0.05; ***P < 0.001.