Fig. 4.

Tracing of Nestin-positive cells in vivo. (A) Nes-CreERT/R26R-eYFP mice were injected with tamoxifen for five consecutive days to induce recombination. Mice were killed at different time points as indicated with asterisks. (B) YFP⁺ cells in the adrenals are indicated with arrows. (Scale bars, 100 µm.) (C) The distance of YFP⁺ cells to the adrenal capsule. *P < 0.05; ***P < 0.001. (D) Number of YFP⁺ cells per cryosection. ***P < 0.001. (E) Quantification of double-positive cells (n ≥ 3 mice per time point, n ≥ 3 cryosections per adrenal). Data in C–E are presented as mean ± SEM. Dashed lines mark the border between the cortex (c) and medulla (m).