Fig. 1.

ACBP is an important element of the low-oxygen stress response pathway in plants. (A) Multiple sequence alignment of class II ACBP ankyrin domain compared with ACBP1 and ACBP2 ankyrin domains in A. thaliana (27). (B) Multiple sequence alignment of ERFVII DNA-binding domain compared with RAP2.12 and RAP2.2 in A. thaliana (26). Sequences of homologous proteins were obtained from Phytozome v12.0 and aligned with Clustal X. (C) Eleven-day-old seedlings of wild type and acbp1 after 9 h of anoxia and 3-d recovery. (Scale bar: 1.0 cm.) (D) Survival scores for wild type and acbp1 after 9-h anoxia and 3-d recovery. Data are mean values ± SD; *P < 0.05, n = 4 (15 seedlings per replicate). (E) Effect of 2 h of hypoxia treatment (1% O₂) on the localization of GFP-tagged ACBP1 in epidermal cells. Representative pictures are shown. (Scale bar: 10 µm.)