Fig. 2.

Application of C18:1-CoA induces RAP2.12 relocalization into the nucleus. (A) Representative Western blot showing in vitro ACBP1:RAP2.12 complex stability after treatment with C18:1-CoA or C16:0-CoA. Pluronic F68 treatment served as control. (B) Quantification of ACBP1-to-RAP2.12-ratio as shown in A. Data are mean values ± SD *P < 0.05, n = 8. (C) Percentage of epidermal cells with nuclear localization of RAP2.12-GFP after treatment with different acyl-CoAs. Data are mean values ± SD; *P < 0.05. (D) Localization of RAP2.12-GFP in detached leaves incubated with 0.1% C18:1-CoA, C18:0-CoA or C16:0-CoA dissolved in 0.01% pluronic F68 under normoxic conditions for 3 h. Treatment with pluronic F68 only served as negative control. DAPI staining was used to identify nuclei. Arrows indicate nuclei with GFP signal. (Scale bar: 10 µm.)