Fig. 3.
Changing oleoyl-CoA levels induces low-oxygen responsive gene expression. (A) Number of significantly differentially expressed genes in leaves after 1.5-h treatment with different acyl-CoAs as determined by RNA-Seq (FDR-adjusted P value <0.05). (B) Number of GO classes in which differentially expressed genes are significantly overrepresented under acyl-CoA treatments as shown in A. (C) qPCR analysis of differential expression of hypoxia-responsive genes after acyl-CoA treatment in air (reference: F68 only). Data are presented as mean values *P < 0.05, n = 5. (D) C18:1-CoA levels increase upon hypoxia in wild type. Data are mean values ± SD; *P < 0.05, n = 3. (E) C16:0-CoA levels decrease upon hypoxia in wild type. Data are mean values ± SD; *P < 0.05, n = 3. (F) C18:1-CoA levels are increased in lacs4lacs9 double mutants grown in air. Data are mean values ± SD; *P < 0.05, n = 3. (G) C16:0-CoA levels are lowered in lacs4lacs9 double mutants. Data are mean values ± SD; *P < 0.05, n = 3. (H) Expression data for hypoxia-responsive genes comparing wild type and lacs4-1 lacs9-2 in air or hypoxia (2 h 1% O2; mean values, *P < 0.05, n = 4).