Fig. 4.
RALF22 overexpressing plants phenocopy lrx345 and fer-4 mutants. (A) qRT-PCR analysis of the transcript levels of RALF22 in WT and two independent RALF22 overexpressing lines (#5 and #6). ACTIN8 was used as the internal control. Values are means ± SD (n = 3); **P < 0.01 (Student’s t test). (B) Rosette morphology (Top) and anthocyanin accumulation (Bottom) of WT and two independent RALF22 overexpressing lines (#5 and #6) grown in soil for 4 wk. (Scale bar, 1 cm.) (C) Phenotypes of WT and RALF22 overexpressing plants (#5 and #6) grown on MS and MS + NaCl (120 mM) media. (D) Survival rates of WT and RALF22 overexpressing plants on MS + NaCl medium (120 mM). Values are means ± SD (n = 3); **P < 0.01 (Student’s t test).