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HIV-1 TAR binding to Tat:AFF4:P-TEFb. Binding of WT TAR and TAR mutants to WT Tat:AFF4:P-TEFb was measured in EMSAs with 100 pM 32P-labeled TAR27. Error bars reflect the SD from three experimental replicates. TAR mutations are indicated in bold letters in the schematic depiction of TAR (right).
