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. 2018 Nov 29;115(51):E11978–E11987. doi: 10.1073/pnas.1810598115

Fig. 6.

Fig. 6.

The EMT negative-feedback loop is mediated by DPYSL3. (A) Box and whiskers plots with center line representing mean mRNA expression levels of SNAI1/2, TWIST1/2, ZEB1/2, and VIM of CLOW breast tumors in the METABRIC dataset (25, 26). Box extends to interquartile range (IQR), and whiskers extend to 1.5 × IQR of mean gene expression. Comparison of gene expression signatures among three ranks (low, middle, or high) of DPYSL3 expression is shown. P value was determined by Kruskal–Wallis test. (B) Western blotting of DPYSL3 in HMLEs expressing control vector, TWIST, and SNAIL. DNA-PKcs was used for a loading control. (C) Western blotting of DPYSL3 in WHIM12 expressing control vector and SNAIL. (D, Left) Western blotting of EMT markers in HMLE-TWIST siControl and HMLE-TWIST siDPYSL3 cells. (D, Right) Quantification on the right from three independent experiments ± SEM. **P < 0.01 (Student t test).