Fig. 1.

The structure of donor plasmid used in embryo injection (a) and expression of the EGFP marker in transgenic mosquitoes (b). a The donor plasmid contains a transformation marker, EGFP driven by the 3xP3 promoter, and a firefly luciferase coding cassette under the control of the 1kb upstream promoter sequence of KLC2 (KLC2-P). The entire sequence of the luciferase reporter cassette is provided as Additional file 6. piggyBac arms are also shown. Arrows indicate primer sets for inverse PCR (iPCR). Due to space limitation, nested iPCR primers are not drawn. All primers are shown in Additional file 3. b Images of transgenic positive larva (L) and pupa (P). Arrow heads point to EGFP marker expression in the eyes