Fig. 3.

Profiles of the luciferase protein activity (a) and transcript level (b) in the early embryos of the JT0311-M3-homo line. a The luciferase assay showed that the luciferase signal was first observed in 3-4 h embryos, peaked in the 4-5 h embryos and disappeared after 9-10 h. b The RT-ddPCR showed that the luciferase transcript was observed in 2-3 h embryos, peaked in 3-4 h embryos and disappeared after 7-8 h. AAEL002401 was used as an internal reference