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Testis expression of the transgene. a The luciferase protein was detected in 1 and 2 day old male pupae (MP-1d, MP-2d) but not in 1 and 2 day old female pupae (FP-1d, FP-2d), male larvae (ML-4instar), or female larvae (FL-4instar), according to luciferase assay. b The luciferase protein activity in adult male was mainly detected in the testes. c RT-ddPCR showed that the luciferase transcripts were mainly detected in the male testes
